Solid-phase nucleic acid isolation

Details Solid-phase nucleic acid isolation Solid-phase nucleic acid isolation

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2289943

The present invention provides a method of isolating nucleic acid from a sample of cells, said method comprising: (a) binding cells in said sample to a solid support to isolate cells from the sample; (b) lysing the isolated cells; and (c) binding nucleic acid released from said lysed cells to said same solid support and a kit for carrying out such a method. '1'he method may advantageously be used to prepare nucleic acid for use in a nucleic acid-based target cell detection method.

La présente invention a trait à un procédé permettant d'isoler l'acide nucléique contenu dans un échantillon de cellules. Ce procédé consiste à (a) fixer les cellules dudit échantillon à un support solide dans le but d'isoler les cellules de l'échantillon, (b) lyser les cellules isolées; et (c) fixer l'acide nucléique libéré par lesdites cellules lysées sur le même support solide. L'invention concerne, par ailleurs, un kit permettant de mettre en oeuvre ce procédé. Ce procédé peut convenir à la préparation d'acide nucléique destiné à être utilisé dans un procédé de détection d'une cellule cible utilisant de l'acide nucléique.

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