Solid phase serology assay

Details Solid phase serology assay Solid phase serology assay

G - Physics

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167/44

G01N 33/543 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2030232

7583 SOLID PHASE SEROLOGY ASSAY ABSTRACT OF THE DISCLOSURE A rapid, sensitive and specific assay for the detection of antibodies to infectious diseases. The method utilizes a porous member to which there is immobilized a binding material capable of binding to the antibodies of a desired subclass or subclasses which are present in the test sample. According to the method, where the test sample includes antibodies to the infectious disease of interest, there is formed a ternary complex of the binding material, the antibody of interest and a labeled detector material, e.g., a labeled antigen, which is specific to the antibody of interest. After removal of the free labeled detector material from the reaction zone any bound labeled detector material is detected to provide an indication of the presence of the antibodies of interest.

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