Solid phase system for ligand assay

Details Solid phase system for ligand assay Solid phase system for ligand assay

C - Chemistry, Metallurgy

12

Q

167/37

C12Q 1/42 (2006.01) G01N 33/543 (2006.01) G01N 33/74 (2006.01)

Patent

CA 1179940

ABSTRACT OF THE DISCLOSURE A method for conducting a ligand assay in an inert porous medium wherein a binding material is immunologically immobilized within the medium, which includes the steps of immunologically immobilizing a binding material within a finite zone of the medium, applying an analyte to the zone containing the immobilized binding material, applying a labeled indicator to the zone which becomes immobilized within the zone in an amount which can be correlated to the amount of analyte in the zone, applying a solvent to substantially the center of the zone to chromatographically separate the unbound labeled indicator from the zone, and measuring the amount of labeled indicator remaining in the zone.

394793

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