Solid phase system incorporating tetrazolium salts for use...

Details Solid phase system incorporating tetrazolium salts for use... Solid phase system incorporating tetrazolium salts for use...

G - Physics

01

N

167/44

G01N 33/543 (2006.01) C12Q 1/68 (2006.01) G01N 33/549 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1289876

H-7321 ABSTRACT A solid phase system incorporating a tetrazolium salt for use in a ligand receptor assay for the detection of a selection analyte in fluid sample is disclosed, wherein the tetrazolium salt functions to augment the color development of the indigogenic enzyme substrates used in such assays. The solid phase system comprises a solid support on which is localized in the same area a receptor and a tetrazolium salt. Methods for localizing the receptor on the solid support include chemical coupling and entrapment of receptor coated microspheres. The tetrazolium salt may be localized on the solid support by adsorption by hydrophobic interaction with the solid support or by entrapment of tetrazolium salt coated microspheres. The solid phase system is useful when incorporated in an apparatus for conducting ligand receptor assay processes. The use of the solid phase system in immunoassays and nucleic acid assays are described.

541349

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