Solid-phase test for the detection of periodontal disease in...

C - Chemistry – Metallurgy – 12 – Q

Patent

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150/16, 150/26

C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) C12Q 1/06 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2008065

Subgingival plaque samples can be analyzed for the presence of the trypsin substrate N-benzoyl-DL-arginine-2-naphthylamide (BANA) in order to make a clinical diagnosis of periodontal disease. The solid phase test utilizes enzyme sensitive reagents adsorbed separately onto two elements. The sample is added to one element, the second element contacted therewith and water is added to initiate the reaction. The resulting analysis is complete within thirty minutes. Positive tests indicating periodontal disease-causing organisms are determined by a color change on the element containing the Fast Black K salt color developer. The test provides a stable, readable, permanent record which can be included in the patient's dental records.

Recherche de N-benzoyl-DL-arginine-2-naphtylamide (BANA), substrat de la trypsine, dans des échantillons de plaque sous-gingivale en vue du diagnostic clinique d'affection périodontique. Le test en phase solide fait appel à des réactifs sensibles aux enzymes adsorbés séparément sur deux éléments. L'échantillon est ajouté à un élément, le deuxième élément est mis en présence du premier et l'eau est ajoutée pour amorce la réaction. L'analyse est réalisée en trente minutes. La présence d'organismes responsables d'affection périodontique est révélée par un changement de couleur de l'élément contenant le révélateur de couleur au sel de potassium (K) Fast Black. Par ce procédé, on obtient un film stable, lisible et permanent qui peut être versé dans la fiche dentaire du patient.

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