Solid support assay systems and methods utilizing...

Details Solid support assay systems and methods utilizing... Solid support assay systems and methods utilizing...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2425747

Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide the solid support. In some instances, the molecular recognition sequence includes one or more non- standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorpoaration of a non-standard base (e.g., via PCR or ligation) is used in the assay.

L'invention concerne des essais biologiques sur support solide à l'aide de bases non normalisées. Un oligonucléotide de capture comprenant une séquence de reconnaissance moléculaire est relié à un support solide puis hybridé avec un oligonucléotide cible du support solide. Dans certains cas, la séquence de reconnaissance moléculaire comprend une ou plusieurs bases non normalisées et elle s'hybride à une séquence de marquage de l'oligonucléotide cible. Dans d'autres cas, on incorpore une base non normalisée (par exemple, par ACP ou par ligature) dans l'essai biologique.

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