C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/122, 195/141
C12P 19/34 (2006.01) C07H 21/00 (2006.01) C12M 1/40 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 1336690
The present invention relates to a support for the immobilization of nucleotide sequences. This support comprises a diol, a controlled pore glass particle and a phosphodiester linkage. The diol has its first hydroxyl group linked to the controlled pore glass particle through bonding to the phosphodiester linkage while its second hydroxyl group is free for linking to a nucleotide. The present invention also relates to a process for synthesizing nucleotide sequences. It first comprises immobilizing a nucleotide fragment to the above-mentioned support by linking nucleoside phosphoramidites to the free hydroxyl group of the diol. Then, a protected nucleotide segment is immobilized onto the support by tandem ligation to the immobilized fragment. Optionally, one or more nucleotide segments are ligated to the free end of the linked segment by tandem ligation. The synthesized nucleotide sequence is then deprotected and the support is submitted to the action of a restriction enzyme to recover the desired nucleotide sequence.
615014
Anderson J. Wayne
Georges Fawzy
LandOfFree
Solid support for immobilizing nucleotide sequences does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Solid support for immobilizing nucleotide sequences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Solid support for immobilizing nucleotide sequences will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1317144