Solubility tags for the expression and purification of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/62 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2689099

Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.

L'invention concerne des marqueurs de peptides, dénommées ici marqueurs de corps d'inclusion, qui sont utiles pour la génération de peptides de fusion insolubles. Les peptides de fusion comprennent au moins un marqueur de corps d'inclusion lié de manière fonctionnelle à un peptide d'intérêt. L'expression du peptide de fusion dans une cellule hôte résulte en un produit qui est insoluble et est contenu au sein des corps d'inclusion dans la cellule et/ou le lysat cellulaire. Les corps d'inclusion peuvent alors être épurés et la protéine d'intérêt peut être isolée après séparation du marqueur de corps d'inclusion.

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