Solubilization of proteins in active forms

Details Solubilization of proteins in active forms Solubilization of proteins in active forms

C - Chemistry, Metallurgy

07

K

C07K 1/107 (2006.01) C07K 1/113 (2006.01) C07K 14/435 (2006.01) C07K 14/61 (2006.01)

Patent

CA 2090701

A method of preparing a protein in a physiologically active form, which method includes providing a source of protein in an at least partially solubilised form; and a refolding agent including an effective amount of a low molecular weight monothiol or derivative thereof; subjecting the solubilised protein to a refolding step, in the presence of the refolding agent.

Procédé de préparation d'une protéine sous une forme physiologiquement active, ledit procédé comprenant l'utilisation d'une source de protéine sous une forme au moins partiellement solubilisée; et d'un agent de réplication comprenant une quantité efficace d'un monothiol de faible masse moléculaire ou d'un dérivé de ce dernier. Dans ce procédé, on soumet la protéine solubilisée à une étape de réplication, en présence de l'agent de réplication.

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