Soluble, active hepatitis c virus protease

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/57 (2006.01) C07K 14/18 (2006.01) C12N 9/50 (2006.01)

Patent

CA 2220575

Soluble HCV NS3 protease, including the NS3 protease fused to a solubilizing motif. A fusion of the NS3 and NS4 regions under conditions where they are not cleaved by the NS3 protease. Bacterially expressed soluble HCV NS3 protease. Host cells wherein at least 1 % of the cell's total protein is soluble HCV NS3 protease.

La présente invention se rapporte à une protéase NS3 soluble du virus de l'hépatite C qui est fusionnée à un motif de solubilisation; à la fusion des régions NS3 et NS4 dans des conditions permettant que celles-ci ne soient pas clivées par la protéase NS3; à la protéase NS3 soluble du virus de l'hépatite C exprimée par voie bactérienne; à des cellules hôtes dans lesquelles au moins 1 % de la totalité de la protéine des cellules est une protéase NS3 soluble du virus de l'hépatite C.

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