Soluble stabilized enzymes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

195/35.2, 150/3.

C12N 9/96 (2006.01) C12N 11/08 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 1177421

Abstract of the Disclosure A method is disclosed for preparing a soluble stable enzyme. The method comprises the steps of reacting in a liquid media an enzyme with a polymer having pendant groups capable of covalently bonding with pendant groups on the enzyme. The enzyme and polymer are mixed with at least one composition which affects the activity of the enzyme. Such a composition can be selected from the enzyme substrate, product of the enzyme substrate reaction, an activator for the enzyme and/or inhibitor for the enzyme. In addition, a composition can be added which can also competitively react with the available pendant groups of the polymer.

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