Solution phase nucleic acid sandwich assay and...

Details Solution phase nucleic acid sandwich assay and... Solution phase nucleic acid sandwich assay and...

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 1317207

-59- ABSTRACT OF THE DISCLOSURE Methods and compositions are provided for rapid detection of nucleic acid sequences. The method employs two reagent sets. The first set is a labeling set comprising: (1) a first nucleic acid sequence probe having an analyte complementary region and a first recognition sequence region and (2) a labeled sequence complementary to the first recognition sequence region. The second set is a capturing set comprising: (1) a second nucleic acid sequence probe having an analyte complementary region and a second recognition sequence region, (2) a specific binding pair member conjugated to a sequence complementary to the second recognition sequence, and (3) a separating means to which is bound a complementary specific binding pair member. The sample and probes are combined under annealing conditions, followed by addition of the other reagents, separation of the bound label from the supernatant and detection of the label in either phase. The invention also encompasses nucleic acid probes formed from one or more modified, derivatizable nucleotides.

616159

Affiliated with

Also associated with

Rating

Solution phase nucleic acid sandwich assay and... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Solution phase nucleic acid sandwich assay and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Solution phase nucleic acid sandwich assay and... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1243979