C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/70 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) F02B 75/02 (2006.01)
Patent
CA 2178598
New techniques are provided for substan- tially reducing background signals encountered in solution phase hybridization assays. The tech- niques are premised on eliminating or signifi- cantly reducing the phenomena of nonspecific hy- bridization and nonspecific binding, so as to pro- vide a detectable signal which is produced only in the presence of the target polynucleotide of interest. In certain embodiments, methods are provided for increasing the signal which can oth- erwise be diminished in noise reduction. Kits for carrying out the novel assays are provided as well.
L'invention concerne de nouvelles techniques pour réduire sensiblement les signaux de fond rencontrés dans des essais d'hybridation en phase de solution. Les techniques se fondent sur la suppression ou la réduction significative des phénomènes d'hybridation non spécifique et de fixation non spécifique afin de fournir un signal pouvant être détecté qui n'est généré qu'en présence du polynucléotide cible concerné. Dans certains modes de réalisation, des procédés sont prévus pour augmenter le signal qui dans d'autres cas, peut être diminué lors de la réduction du bruit. L'invention concerne également des kits pour effectuer ces nouveaux essais.
Collins Mark
Fultz Timothy
Urdea Michael S.
Warner Brian D.
Borden Ladner Gervais Llp
Corporation Bayer
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