Solvent extraction for separation in immunoassay

Details Solvent extraction for separation in immunoassay Solvent extraction for separation in immunoassay

G - Physics

01

N

167/37

G01N 33/537 (2006.01)

Patent

CA 1140463

ABSTRACT OF THE INVENTION The present invention relates to a simple and accurate method to be applied in immunoassay technique for separating the free ligand from the antibody-bound ligand. The method involves the use of a solvent extraction operation which freezes the equilibrium between the free- and bound-fraction. The solvent to be utilized should be slightly water miscible or completely water immiscible, having the properties of marked selective extraction power towards the antigen. The method is applicable in a liquid-liquid system, a solid-liquid system and solid-solid system. The method is useful for radioimmunoassay, free radical assay or metallo-immunoassay, being most versatile having also the advantage that as a result of the free ligand being extracted into the solvent, the determination of the free and/or bound fraction compares very favourably with the known prior are methods in immunoassays.

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