G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/82 (2006.01) C07K 16/44 (2006.01)
Patent
CA 2488240
The invention discloses monoclonal antibodies for vitamins A (retinol palmitate) and D3 (cholecalciferol); a method for using monoclonal antibodies, and the monoclonal antibodies disclosed herein, in particular, to quantitate these vitamins in fluids such as dairy products, and blood, and also raw or processed agri-food and beverage products. The method involves contacting the sample with a mixture of polar and non-polar organic solvents in combination with inorganic salts to remove fat molecules into an organic fraction, and assaying the organic fraction by immunoassay involving the monoclonal antibodies. The presence of a mixture of non-polar and polar organic solvents increases the separation of vitamins from fat molecules and enables the test samples to be quantified by immunoassay without any further treatment.
L'invention concerne des anticorps monoclonaux pour les vitamines A (palmitate de rétinol) et D3 (cholécalciférol); un procédé d'utilisation des anticorps monoclonaux, et ces anticorps monoclonaux, notamment afin de quantifier ces vitamines dans des fluides tels que les produits laitiers, et le sang, ainsi que les produits agro-alimentaires bruts ou traités et les boissons. Ce procédé consiste à mettre en contact l'échantillon avec un mélange de solvants organiques polaires et non polaires en combinaison avec des sels inorganiques afin d'enlever les molécules lipidiques sous forme de fraction inorganique, et à doser la fraction organique par dosage immunologique impliquant les anticorps monoclonaux. La présence d'un mélange de solvants non polaires et polaires augmente la séparation des vitamines des molécules lipidiques et permet la quantification des échantillons tests par dosage immunologique sans autre traitement.
Bennett Jones Llp
Scimed Laboratories Inc.
Scimed Technologies Inc.
LandOfFree
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