Solvent vehicles for reagent delivery in oligonucleotide...

Details Solvent vehicles for reagent delivery in oligonucleotide... Solvent vehicles for reagent delivery in oligonucleotide...

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) B01J 19/00 (2006.01) C11D 7/50 (2006.01)

Patent

CA 2274534

Solvent vehicles and methods of their use in the pulse jet delivery of reagents in automated oligonucleotide synthesis by the solid-phase phosphoramidite method are provided. For pulse jet delivery of the activated monomer, acetonitrile in combination with at least one stabilizer co-solvent is employed as the solvent vehicle, where the stabilizer co-solvent is an inert, organic solvent having a boiling point of at least 100 ~C. For pulse jet delivery of the detritylation reagent, a mono- or disubstituted methane is employed as the solvent vehicle, where substituents have a molecular weight greater than 36 daltons. Use of the solvent vehicles according to the subject invention provides for reduced reagent precipitation in the pulse jet tubes and improved results.

Véhicules de solvant et leurs procédés d'utilisation dans la distribution par jets pulsés de réactifs lors de la synthèse automatisée d'oligonucléotides au moyen du procédé faisant intervenir des phosphoramidites en phase solide. Pour la distribution par jets pulsés du monomère activé, on utilise de l'acétonitrile en combinaison avec au moins un cosolvant stabilisant, en tant véhicule de solvant. Le cosolvant stabilisant est un solvant organique inerte présentant un point d'ébullition d'au moins 100 ·C. Pour la distribution par jets pulsés du réactif de détritylation, un méthane mono ou disubstitué est utilisé comme véhicule de solvant, les substituants présentant un poids moléculaire supérieur à 36 daltons. L'utilisation de véhicules de solvant selon l'invention permet de réduire la précipitation de réactifs dans les tubes à jets pulsés et d'obtenir ainsi de meilleurs résultats.

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