Somatic cloning gene transfer for producing recombinant...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) A61K 35/12 (2006.01) A61K 35/28 (2006.01) A61K 38/17 (2006.01) A61K 48/00 (2006.01) C07K 16/06 (2006.01) C07K 16/30 (2006.01) C07K 16/46 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2416877

The invention relates to a method for recombinant production of substances, wherein cells are transformed with a nucleotide sequence coding said substance, the transformed cells are cloned and the cells thus obtained are inserted into a receiving organism. More particularly, the invention relates to the use of said method in the production of recombinant proteins, cells and tissues. The invention further relates to a method wherein cells of an individual are isolated, said cells are inserted into an immune-incompetent animal for further growth and the cells, tissue and/or organs which have grown in the animal are re-isolated and inserted into an individual.

L'invention concerne un procédé pour produire des matières par recombinaison, selon lequel des cellules sont transformées par une séquence nucléotidique codant la matière concernée, ces cellules transformées sont soumises à un processus de clonage et les cellules ainsi obtenues sont introduites dans un organisme receveur. La présente invention concerne notamment l'application dudit procédé lors de la production par recombinaison de protéines, de cellules et de tissus. Cette invention porte également sur un procédé selon lequel des cellules d'un individu sont isolées, puis introduites dans un animal sans immunité pour qu'elles se multiplient. Les cellules, tissus et/ou organes ainsi développés dans l'animal sont ensuite isolés pour être introduites dans un individu.

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