Somatotropins with alterations in the alpha-helix 1 region,...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/61 (2006.01) C07K 1/113 (2006.01)

Patent

CA 2056467

The present invention relates to somatotropin analogues with amino acid changes in the alpha-helix 1 regions of said somatotropins, alone or in combination with mutations in the alpha-helix 3 and/or alpha-helix 2 regions, plus combinations with other changes to the native amino acid sequence of somatotropins. The resulting analogues are stable for formulation in sustained release formulations, while maintaining biological activity. Further, methods for conducting site-directed mutagenesis on DNA encoding proteins and/or polypeptide also are provided.

La présente invention traite d'analogues de la somatotropine avec des changements d'acides aminés dans les régions 1 de l'hélice alpha des somatotropines en question, seuls ou en combinaison avec des mutations dans les régions 3 et(ou) 2 de l'hélice alpha, avec, en outre, des combinaisons aveec d'autres changements à la séquence naturelle d'acides aminés des somatotropines. Les analogues résultants sont stables dans des formulations à libération lente et gardent leur activité biologique. Des méthodes pour effectuer une mutagenèse à des sites précis sur l'ADN codant pour des protéines et(ou) des polypeptides, sont également fournies.

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