Soybean cysteine proteinase inhibitors, nucleotides encoding...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 14/81 (2006.01) C12N 15/82 (2006.01) A01N 65/00 (2006.01)

Patent

CA 2247798

The invention relates to the isolation, purification and use of three soybean cysteine proteinase inhibitors, designated L1, R1 and N2, which have strong inhibitory activity with respect to cysteine proteinases found in the digestive tracts of certain herbivorous insects. The invention further relates to three nucleotide sequences which have been cloned, isolated and sequenced, these nucleotide sequences coding for the three above-named proteins, as well as a vector having one or more of these sequences provided therein. The invention also relates to plants transformed by inventive vectors, transformed plants having an enhanced ability to resist predation by said insects, as well as microorganisms transformed using inventive vectors or plasmids.

Cette invention se rapporte à l'isolation, à la purification et à l'utilisation de trois inhibiteurs de cystéine-protéinases du soja, appelé L1, R1 et N2, qui ont un fort pouvoir inhibiteur sur les cystéine-proteinases se trouvant dans l'appareil digestif de certains insectes herbivores. Cette invention se rapporte en outre à trois séquences nucléotidiques qui ont été clonées, isolées et séquencées, ces séquences nucléotidiques codant pour ces trois protéines mentionnées, ainsi qu'un vecteur dans lequel on a introduit une ou plusieurs de ces séquences. Cette invention se rapporte également à des plantes transformées par des vecteurs faisant l'objet de cette invention, à des plantes transformées ayant une capacité accrue de résister à la prédation par ces insectes, ainsi qu'à des micro-organismes transformés au moyen de vecteurs ou de plasmides de cette invention.

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