Soybean event 127 and methods related thereto

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01N 25/32 (2006.01) A01N 43/50 (2006.01) A01P 13/00 (2006.01) C12M 1/34 (2006.01) C12N 9/10 (2006.01) C12N 15/00 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2748973

Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location may comprise genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO: 5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and en-ables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.

L'invention porte sur des compositions et des procédés liés à des plants de soja transgéniques résistants à des herbicides inhibant AHAS. L'invention porte également sur des plants de soja d'évènement 127 ayant une séquence codant pour AHAS mutée qui confère une tolérance à un herbicide inhibant AHAS. Les plants de soja d'évènement 127 ayant la molécule d'acide nucléique d'évènement 127 au niveau de la position chromosomique identifiée peuvent comprendre des jonctions génomiques/transgéniques ayant au moins la séquence d'acide nucléique de SEQ ID NO: 5 et/ou 6. La caractérisation du site d'introduction génomique de l'évènement 127 fournit un rendement de sélection amélioré et permet l'utilisation de marqueurs moléculaires pour suivre l'insert de transgène dans les populations en sélection et leur descendance. L'invention porte également sur divers procédés et compositions pour l'identification, la détection et l'utilisation des plants de soja d'évènement 127.

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