Soybean event dp-305423-1 and compositions and methods for...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 1/04 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2666754

Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.

L'invention concerne des compositions et des procédés relatifs à des plants de soja transgéniques tolérants à l'acide oléique/inhibiteur d'ALS. La présente invention concerne plus spécifiquement des plants de soja ayant un événement DP-305423-1 qui confère un phénotype à teneur élevée en acide oléique et une tolérance à au moins un herbicide inhibiteur d'ALS. Le plant de soja possédant l'évènement DP-305423-1 contient des jonctions génomiques/transgéniques possédant au moins la séquence polynucléotide de SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. La caractérisation du site d'insertion génomique de l'évènement DP-305423-1 offre une meilleure efficacité de reproduction et permet l'utilisation de marqueurs moléculaires afin de tracer l'insertion de transgènes dans les populations de reproduction et leur descendance. L'invention concerne également divers procédés et diverses compositions d'identification, de détection, et d'utilisation des événements de soja DP-305423-1.

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