Soybean transformation method

Details Soybean transformation method Soybean transformation method

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01)

Patent

CA 2359868

A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.

L'invention concerne un procédé de transformation génétique des cellules germinales du soja par l'intermédiaire de Agrobacterium. Ce procédé repose sur l'introduction par l'intermédiaire de Agrobacterium de gènes dans les cellules individuelle du méristème du soja fraîchement germé, ces cellules pouvant être activées de manière à former directement des pousses donnant des plantes transgéniques. Ce procédé est rapide et efficace et ne comprend pas de culture de tissus en phase de cal.

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