Sparger and apparatus for and method of growing cells

B - Operations – Transporting – 01 – F

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195/145, 195/45

B01F 3/06 (2006.01) B01F 3/04 (2006.01) B01F 5/06 (2006.01) C12M 3/00 (2006.01)

Patent

CA 1275272

ABSTRACT OF THE DISCLOSURE A sparger useful for introducing gas into a liquid is composed of one or more gas-permeable members positioned between a gas inlet means and a gas distribution means. A method and apparatus for culturing cells, eukaryotic cells and hybridomas in particular, wherein circulation of a liquid medium contained in the apparatus is achieved by gas introduced into the liquid medium by means of the aforementioned sparger.

501108

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