C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
260/228, 260/233
C07H 17/06 (2006.01) C07D 209/48 (2006.01) C07D 233/78 (2006.01) C07H 17/075 (2006.01) C07H 21/00 (2006.01) G01N 33/533 (2006.01) G01N 33/535 (2006.01)
Patent
CA 1133474
ABSTRACT OF THE DISCLOSURE An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula: G-D-R wherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glyco- sidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or hetero- geneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens such as drugs, particularly the aminoglycoside antibiotics.
320897
Boguslaski Robert C.
Burd John F.
Carrico Robert J.
Miles Laboratories Inc.
Osler Hoskin & Harcourt Llp
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