Specific binding assay employing anti-g6pdh as label

Details Specific binding assay employing anti-g6pdh as label Specific binding assay employing anti-g6pdh as label

G - Physics

01

N

167/45, 150/3.3

G01N 33/573 (2006.01) C12Q 1/32 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1237967

ABSTRACT OF THE DISCLOSURE A specific binding assay method and reagent system based on the use of an inhibitory anti-enzyme, e.g., antibody or fragment thereof, as the label component. Such method and reagent system have been improved by selection of anti-(glucose-6-phosphate dehydrogenase) [anti-G6PDH] as the anti-enzyme label. The improved label is monitored by its ability to inhibit G6PDH. The resulting assay is more sensitive, requires lesser quantities of reagents, is less sus- ceptible to sample interferences, and employs a reagent system having greater stability than the pub- lished prior art method employing an anti-peroxidase label. The present invention is particularly applicable to homogeneous immunoassays for determining substances appearing at low concentrations in biological fluids such as urine and serum.

466799

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