Specific binding assay employing polyvinyl alcohol as...

Details Specific binding assay employing polyvinyl alcohol as... Specific binding assay employing polyvinyl alcohol as...

G - Physics

01

N

150/11, 167/37

G01N 33/53 (2006.01) G01N 33/50 (2006.01) G01N 33/538 (2006.01)

Patent

CA 1108986

ABSTRACT OF THE DISCLOSURE A specific binding assay method and test kit for deter- mining a ligand, particularly an antigen or hapten, in, or the ligand binding capacity of, a liquid medium, particularly a body fluid such as serum, wherein the liquid medium is combined with assay reagent means including a labeled binding component such as a radiolabeled form of the ligand, with the improvement that separation of the resulting bound species and free-species of the labeled component is accom- plished by selectively adsorbing the free-species onto a solid comprising a nonion-exchange cross-linked polyvinyl alcohol. The method is particularly suited to the radioimmunoassay detection of nonproteinaceous haptens such as thyroxine, triiodothyronine, estriol, vitamin B12 and digoxin. The polyvinyl alcohol adsorbent provides a very effective and readily available separation means for use in specific bind- ing assays.

291089

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