Specific binding flow cytometry method

Details Specific binding flow cytometry method Specific binding flow cytometry method

G - Physics

01

N

167/45

G01N 33/48 (2006.01) G01N 15/14 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1254134

ABSTRACT A flow cytometry method for reproducibly detecting and counting a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color- producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.

476638

Affiliated with

Also associated with

Rating

Specific binding flow cytometry method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Specific binding flow cytometry method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Specific binding flow cytometry method will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1202318