Specific binding members against synaptophysin

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/13 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) A61K 49/00 (2006.01) A61K 51/10 (2006.01) A61P 1/16 (2006.01) C07K 16/00 (2006.01) C07K 16/28 (2006.01) C12P 21/08 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) C07K 14/705 (2006.01)

Patent

CA 2584608

The present invention provides specific binding members that bind synaptophysin and which comprise: an antibody VH domain selected from the group consisting of the C1-3 VH domain (SEQ ID NO. 2) and a VH domain comprising a VH CDR3 with the amino acid sequence of SEQ ID NO. 12 and optionally one or more VH CDR's with an amino acid sequence selected from SEQ ID NO. 10 and SEQ ID NO. 11; and/or an antibody VL domain selected from the group consisting of the C1-3 VL domain (SEQ ID NO. 4) and a VL domain comprising one or more VL CDR's with an amino acid sequence selected from SEQ ID NO. 13, SEQ ID NO. 14 and SEQ ID NO. 15. The invention further provides related materials such as nucleic acids, kits and compositions, and also methods of use of the binding member, for instance in targeting entities to hepatic stellate cells which are implicated in liver fibrosis.

L'invention concerne des organes de liaison spécifiques qui lient la synaptophysine et qui comprennent : un domaine VH d'anticorps choisi dans le groupe composé du domaine VH C1-3 (SEQ ID NO. 2) et d'un domaine VH comprenant une CDR3 de VH avec la séquence d'acides aminés SEQ ID NO. 12 et, facultativement, une ou plusieurs CDR de VH avec une séquence d'acides aminés choisie entre SEQ ID NO. 10 et SEQ ID NO. 11; et/ou un domaine VL d'anticorps choisi dans le groupe composé du domaine VL C1-3 (SEQ ID NO. 4) et d'un domaine VL comprenant une ou plusieurs CDR de VL avec une séquence d'acides aminés choisie entre SEQ ID NO. 13, SEQ ID NO. 14 et SEQ ID NO. 15. L'invention se rapporte en outre à des matériaux associés tels que des acides nucléiques, des trousses et des compositions, et à des procédés d'utilisation de l'organe de liaison, par exemple pour cibler des entités sur des cellules de Kupffer qui sont impliquées dans la fibrose du foie.

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