C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/57 (2006.01) A61K 38/46 (2006.01) A61P 31/04 (2006.01) C12N 9/14 (2006.01) C12N 9/48 (2006.01) C12N 9/78 (2006.01) C12N 15/55 (2006.01)
Patent
CA 2661896
The Staphylococcus aureus bacteriophage phil 1 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and a SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis-causing pathogens, S. aureus and coagulase negative staphylococci (S. chronogenes, S. epidermis, S. hyicus, S. simulans, S. wameri, and S. xylocus), making it a strong antimicrobial protein and an effective candidate for treating multidrugresistant staphylococci. Lytic activity is maintained at the pH (6.7) and the 'free' calcium concentration (3mM) of milk. Truncated endolysin-derived proteins, containing just the endopeptidase domain, also lyse staphylococci, in the absence of the SH3b- binding domain.
L'endolysine du bactériophage phi 11 de Staphylococcus aureus a deux domaines de peptidoglycane hydrolase (endopeptidase et amidase) et un domaine de liaison à la paroi cellulaire SH3b. Dans des dosages de réduction du trouble, la protéine purifiée peut lyser les pathogènes staphylococciques non traités, provoquant la mastite, S. aureus et les Staphylocoques coagulase négatifs (S. chronogenes, S. epidermis, S. hyicus, S. simulans, S. wameri, et S. xylocus), ce qui en fait une protéine antimicrobienne puissante et un candidat efficace pour le traitement des Staphylocoques multi-résistants. L'activité lytique est maintenue au pH (6,7) et à la concentration en calcium 'libre' (3 mM) du lait. Des protéines tronquées, dérivées d'endolysine, contenant seulement le domaine d'endopeptidase, lysent également les Staphylocoques, en l'absence du domaine de liaison SH3b.
Borden Ladner Gervais Llp
The United States Of America As Represented By The Secretary Of Agricul Ture
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