Specific microbial strain detection method

Details Specific microbial strain detection method Specific microbial strain detection method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2055302

ABSTRACT A method of detecting a particular microbial strain amongst closely related microbial strains and other strains present in vast numbers is described. Following transposition a unique DNA sequence comprising an endogenous transposable element and its flanking DNA sequence is generated. This new sequence is an acquired characteristic, diagnostic for that strain and its progeny alone. In one example of this method, DNA primers are chosen from a region which includes the uniquely located transposable element. These primers are used to amplify the DNA for its subsequent detection, and hence the detection of the particular microbial strain.

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