Spectrophotometer for dual mode fluorescence analysis

G - Physics – 01 – N

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G01N 21/01 (2006.01) F21V 8/00 (2006.01) G01J 3/02 (2006.01) G01J 3/44 (2006.01) G02B 6/35 (2006.01) G02B 26/04 (2006.01) G01J 3/28 (2006.01)

Patent

CA 1053925

SPECTROPHOTOMETER FOR DUAL MODE FLUORESCENCE ANALYSIS Abstract of the Disclosure A sprectrophotometer includes a wide band radiant energy source and a detector for providing an output signal proportional to the intensity of the radiant energy received thereby. A light pipe system is divided into a reference path and a sample path. Such system coacts with a rotary assembly having a variable monochromatic filter located diametrically opposite to an opening through which radiant energy can freely pass. The remaining portions of the rotary assembly are opaque so that as the assembly turns, the sample and reference paths are serially energized by monochromatic energy and then by polychoromatic energy. The sample path includes a sample illumination and collection system for illuminating a test sample and collecting the light reflected therefrom. The resultant output of the detector can be analyzed to determine the amount of fluorescence.

262459

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