Sperm suspensions for sorting into x or y chromosome-bearing...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/076 (2010.01) A61K 35/52 (2006.01) G01N 1/30 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2561661

Sperm cell suspensions comprising a motility inhibitor are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for forming such cell suspensions, as well as processes for staining sperm cells, are also disclosed.

La présente invention concerne des suspensions à spermatozoïdes comprenant un inhibiteur de motilité. Les spermatozoïdes contenus dans de telles suspensions ont une propension à mieux supporter les diverses opérations de tri en fonction de la richesse de leurs populations dans l'un au l'autre des sexes. Il en résulte des compositions triées présentant un nombre accru de spermatozoïdes viables ou mobiles. L'invention concerne également des procédés pour la réalisation de telles suspensions de spermatozoïdes, ainsi que des procédés de coloration des spermatozoïdes.

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