Spherical protein particles and methods of making and using...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/11 (2006.01) A61K 9/14 (2006.01) A61K 9/16 (2006.01) A61K 31/7088 (2006.01) A61K 39/395 (2006.01) A61K 47/30 (2006.01) A61K 48/00 (2006.01) A61K 49/00 (2006.01) C07K 1/22 (2006.01) C07K 14/76 (2006.01) C07K 16/18 (2006.01) C07K 16/46 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2451185

This invention relates to SPPs, spherical nanocrystalline composite particles or crystalline SPPs of biologically active proteins or compositions, including formulations, comprising such SPPs, spherical nanocrystalline composite particles or crystalline SPPs. More particularly, methods are provided for the production of SPPs, spherical nanocrystalline composite particles or crystalline SPPs of high concentration of biologically active proteins, and for the preparation of stabilized SPPs, spherical nanocrystalline composite particles or crystalline SPPs for use alone, or in dry or slurry compositions. This invention also relates to methods for stabilization, storage and delivery of biologically active proteins using SPPs, spherical nanocrystalline composite particles or crystalline SPPs. The present invention further relates to methods using SPPs, spherical nanocrystalline composite particles or crystalline SPPs, or compositions or formulations comprising them, for biomedical applications, including biological delivery to humans and animals. More particularly, SPPs, spherical nanocrystalline composite particles or crystalline SPPs are used as a carrier-free delivery system which can slowly release biologically active proteins to a subject, where and when they are needed.

Cette invention concerne des SPP, des particules composites nanocristallines sphériques, ou des SPP cristallines de protéines ou de compositions biologiquement actives, qui comprennent des préparations contenant de tels SPP, particules composites nanocristallines sphériques ou SPP cristallines. L'invention concerne plus particulièrement des méthodes de production de SPP, de particules composites nanocristallines sphériques ou de SPP cristallines présentant une concentration élevée en protéines biologiquement actives; et des méthodes de préparation de SPP, de particules composites nanocristallines sphériques ou de SPP cristallines stabilisées pouvant être utilisées seules ou dans des compositions en poudre ou en suspension. L'invention concerne aussi des méthodes de stabilisation, d'entreposage et d'administration de protéines biologiquement actives mettant en oeuvre des SPP, des particules composites nanocristallines sphériques ou des SPP cristallines. Elle concerne également des méthodes qui mettent en oeuvre des SPP, des particules composites nanocristallines sphériques ou des SPP cristallines, ou des compositions ou préparations contenant ces éléments, aux fins d'applications biomédicales, y compris l'administration biologique à des humains ou des animaux. Plus particulièrement, les SPP, particules composites nanocristallines sphériques ou SPP cristallines de l'invention sont utilisées comme système d'administration exempt de support pouvant libérer lentement des protéines biologiquement actives chez un sujet, à l'endroit et au moment choisis.

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