C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/071 (2010.01) C12N 5/073 (2010.01) C12N 5/078 (2010.01) A61K 35/12 (2006.01) C07K 14/765 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2373391
A mixture or substance having a spheroid-forming activity is obtained by heat treating fetal calf serum at a temperature and for a period sufficient to impart spheroid-forming activity. Introduced into cell culture, the substance or mixture so obtained causes cells to grow in three-dimensional cultures as opposed to mono-layer. Also disclosed are kits for the production of said mixture or substance and further uses of said mixture or substance.
La présente invention concerne un mélange ou une substance ayant une activité de formation sphéroïde, obtenu par traitement thermique de sérum embryonnaire de veau à une température et pendant une durée suffisantes pour que l'activité de formation sphéroïde se produise. L'introduction de la substance ou du mélange ainsi obtenu dans une culture cellulaire provoque la croissance des cellules dans des cultures tridimensionnelles, contrairement aux cultures monocouches. Cette invention concerne également des équipements destinés à la production dudit mélange ou de ladite substance et leurs utilisations.
Jones Derek
Mcfadden Fincham
University Of Wales College Of Medicine
LandOfFree
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