C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12N 5/07 (2010.01) A61K 39/00 (2006.01) C07K 14/285 (2006.01) C07K 14/47 (2006.01) C07K 14/505 (2006.01) C07K 14/73 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/866 (2006.01)
Patent
CA 2346497
Disclosed and claimed is a new insect cell line, Sf900+, ATCC CRL-12579. The insect cell line was established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1771) through multiple rounds of limiting dilution and selection in a serum-free insect medium supplemented with added human insulin. The insect cell line is useful in BEVS or as an adjuvent and has many characteristics and advantages. Also disclosed and claimed are recombinant proteins from recombinant baculovirus expression in insect cells such as Sf900+ cells, for instance, HA, NA, EPO, CD4, CEA, and thrombospondin.
L'invention porte sur une nouvelle lignée de cellules d'insectes, la Sf900+, ATCC CRL-12579, obtenue à partir de Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1771) par une suite de dilutions limitatrices et de sélections dans un milieu d'insectes additionné d'insuline humaine. Cette lignée de cellules d'insectes est utile dans le BEVS (système d'expression du baculovirus) ou comme adjuvant présente de nombreuses caractéristiques et avantages. L'invention porte également sur des protéines de recombinaison provenant de l'expression de baculovirus dans des cellules d'insectes telles que les cellules Sf900+, par exemple HA, NA, EPO, CD4, CEA, et thrombospondine.
Debartolomeis James
Foellmer Harald G.
Knell John
Smith Gale E.
Voznesensky Andrei I.
Protein Sciences Corporation
Smart & Biggar
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