Spray delivery of cells

Details Spray delivery of cells Spray delivery of cells

A - Human Necessities

61

K

A61K 35/12 (2006.01) C12N 5/071 (2010.01) A61K 35/36 (2006.01) A61L 27/34 (2006.01) A61L 27/38 (2006.01) A61L 27/60 (2006.01) C12N 11/02 (2006.01)

Patent

CA 2352810

Provided is, among other things, a method of adhering cells to a target surface which is a tissue surface of a mammal or such a tissue surface coated with a biodegradable polymer sheet, comprising: coating the target surface with a mixture of a first component comprising a non-polymeric fibrin-related protein and a second component effective for converting the fibrin-related protein to fibrin polymer; and spraying a suspension of the cells onto the coated target surface, wherein the mixed two components have formed a fibrin polymer with a tack effective to adhere the cells.

L'invention concerne une technique permettant de faire adhérer des cellules sur une surface cible, qui est une surface tissulaire d'un mammifère ou une surface tissulaire recouverte d'une feuille polymère biodégradable. Cette technique consiste à recouvrir une surface cible avec un mélange d'un premier composant comprenant une protéine non polymère apparentée à une fibrine, et d'un second composant efficace pour convertir la protéine apparentée à une fibrine en un polymère fibrineux; à pulvériser une suspension de cellules sur la surface cible recouverte, les deux composants mélangés formant un polymère fibrineux présentant un collant efficace pour faire adhérer les cellules sur une surface cible.

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