Stabilisation of double-stranded nucleic acids using proteins

Details Stabilisation of double-stranded nucleic acids using proteins Stabilisation of double-stranded nucleic acids using proteins

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 1/06 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2475949

A composition for treating a nucleic acid duplex, wherein the composition is capable of inhibiting denaturation of the duplex, characterised in that the composition comprises a ubiquitin-like protein and/or a macroglobulin. It has been found that ubiquitin-like proteins (e.g. ubiquitin, NEDD8, RAD23 etc.) and macroglobulins (e.g. .alpha.2-macroglobulin) are able to stabilise nucleic acid duplexes. A nucleic acid duplex which has been contacted with the composition of the invention can be subjected to more stringent processing conditions, with denaturation of the duplex being inhibited, than would otherwise be possible. Corresponding methods and uses are also provided.

L'invention concerne une composition de traitement d'un duplex d'acides nucléiques pouvant inhiber la dénaturation du duplex. Cette composition est caractérisée en ce qu'elle renferme une protéine similaire à l'ubiquitine et/ou une macroglobuline. Selon la présente invention, des protéines similaires à l'ubiquitine (<i>par ex.</i> ubiquitine, NEDD8, RAD23<i>etc.</i>) et des macroglobulines (<i>par ex.</i> .alpha.<sb>2</sb>-macroglobuline) sont capables de stabiliser des duplexes d'acides nucléiques. On peut soumettre un duplex d'acides nucléiques qu'on a mis en contact avec la composition susmentionnée à des conditions de traitement plus rigoureuses, ce qui permet l'inhibition de la dénaturation du duplex qui, autrement, ne serait pas possible. Cette invention concerne aussi des utilisations et des procédés associés.

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