Stabilised reagent and method for determining creatine kinase

Details Stabilised reagent and method for determining creatine kinase Stabilised reagent and method for determining creatine kinase

C - Chemistry, Metallurgy

12

Q

C12Q 1/50 (2006.01)

Patent

CA 2312758

A stabilised reagent for photometric enzymatic determination of creatine kinase (CK) in biological sample material by forming ATP from creatine phosphate and ADP and detection of the ATP thus formed containing an organic or inorganic sulphur compound, preferably with a molar deficiency in relation to the added CK activator. A corresponding reagent remains stable at 2-8 ~C for up to 12 months without incurring any significant functional loss.

L'invention concerne un réactif stabilisé pour la détermination photométrique enzymatique du taux de créatine-kinase (CK) dans un échantillon biologique, par formation d'ATP à partir de phosphocréatine et d'ADP et détection de l'ATP formé contenant un composé sulfuré, de préférence avec un déficit molaire par rapport à l'activateur de CK ajouté. Un réactif correspondant, sous forme liquide, est stable à une température de 2 à 8 ·C, pendant une période pouvant aller jusqu'à douze mois, sans présenter de pertes fonctionnelles importantes.

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