Stabilization of a mutant microorganism population

Details Stabilization of a mutant microorganism population Stabilization of a mutant microorganism population

C - Chemistry, Metallurgy

12

N

195/45

C12N 1/36 (2006.01) C12N 1/38 (2006.01) C12N 15/01 (2006.01) C12P 1/00 (2006.01) C12P 7/44 (2006.01)

Patent

CA 1198071

ABSTRACT OF THE DISCLOSURE This invention provides a process for stabilizing a population of a mutant microorganism in a bioconversion system whereby growth of revertant cells is suppressed. The process involves limiting a nutrient which is essential for cell growth so that the cells selectively grow on a growth carbon source rather than on a non-growth carbon source which is present. The revertant cells being suppressed have a similar ability as the parent strain of the mutant microorganism to grow on the non-growth carbon source in the bioconversion system. The non-growth carbon source in the bioconversion system is metabolized to an extracellular accumulating quantity of a desired metabolite, e.g., toluene is converted to muconic acid.

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