C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/35
C12P 19/02 (2006.01) C12N 9/08 (2006.01) C12N 9/96 (2006.01) C12N 11/02 (2006.01) C12N 11/18 (2006.01)
Patent
CA 1245587
Abstract of the Disclosure Catalse and pyranose-2-oxidase are stabilized according to the invention by means of chemical treatment which render the enzymes resistant to thermal inactivation or inactivation of glucosone or both. Stabilized catalse crosslinked with diimido esters, such as dimethyl suberimidate and dimethyl adipimidate are claimed. The method for stabilizing catalase against thermal inactivation or glucosone inactivation or both comprising gradually adding a crosslinking agent to a catalse solution and maintained pH and temperature control is claimed. Stabilized pyranose-2-oxidase amidinated with an amidinating agent such as ethyl acetimidate is claimed. A method for stabilizing catalase against thermal inactivation comprising gradually adding an amidinating agent to a pyranose-2-oxidase solution preferably with pH control is claimed. An improved process for producing glucosone from glucose using stabilized catalase or pyranose-2-oxidase or both according to the invention is also claimed.
490312
Shaked Ze'ev
Wolfe Sidney N.
Borden Ladner Gervais Llp
Novartis Vaccines And Diagnostics Inc.
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