Stabilization of extracellular enzymes

Details Stabilization of extracellular enzymes Stabilization of extracellular enzymes

A - Human Necessities

61

L

195/139, 195/35.

A61L 33/00 (2006.01) A61K 31/20 (2006.01) C11C 3/02 (2006.01) C12N 9/96 (2006.01) C12N 11/04 (2006.01) C12N 11/08 (2006.01) C12P 7/64 (2006.01) C12P 19/24 (2006.01)

Patent

CA 1280380

ABSTRACT Extracellular enzymes are stabilized with a carboxyalkylated or phosphonoalkylated polymer having a molecular weight of at least 500 Daltons. Exemplified is the stabilization of the enzyme glu- cose isomerase used in a process to convert D-glucose to D-fructose. In a preferred embodiment of the invention the feedstream containing the substrate is contacted initially with the stabilizer and then with the enzyme. In this system the stabilizer and enzyme are maintained in separate reactors. This separation, advantageously, results in a higher half-life for the enzyme.

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