Stabilization of linear double-stranded dna in the presence...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/68 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 9/16 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2496146

The present invention is directed at stabilizing linear polynucleotides against exonucleolytic attack. Adding to both ends of a double-stranded linear DNA a target sequence for a DNA-binding protein the double-stranded linear DNA with the two added target sequences has an increased stability against exonucleolytic attack when contacted with the DNA-binding protein.

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