Stabilization of t4 endonuclease v

Details Stabilization of t4 endonuclease v Stabilization of t4 endonuclease v

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C12N 9/10 (2006.01) C12N 9/22 (2006.01) C12N 9/96 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 2461019

The invention provides T4 endonuclease V compositions that exhibit enhanced stability, including stability at non-refrigerated temperatures, through reduced activity of cryptic proteases. Methods for detecting cryptic protease activity and methods for reducing such activity are also provided.

La présente invention concerne des compositions à base d'endonucléase V des T4 faisant preuve d'une stabilité accrue, notamment aux températures ambiantes non réfrigérées, grâce à une réduction de l'activité des protéases cryptiques. L'invention concerne également des procédés permettant de détecter l'activité des protéases cryptiques ainsi que des procédés visant à réduire une telle activité.

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