Stabilized aptamers to psma and their use as prostate cancer...

C - Chemistry – Metallurgy – 07 – H

Patent

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C07H 21/04 (2006.01)

Patent

CA 2600418

The present invention provides stabilized, high affinity nucleic acid ligands to PSMA. Methods for the identification and preparation of novel, stable, high affinity ligands to PSMA using the SELEX~ method with 2'-O-methyl substituted nucleic acids, and cell surface SELEX~ are described herein. Also included are methods and compositions for the treatment and diagnosis of disease characterized by PSMA expression, using the described nucleic acid ligands.

L'invention concerne des ligands d'acide nucléique stabilisés et d'affinité élevée d'antigènes prostatiques spécifiques membranaires (APSM). L'invention concerne également des procédés d'identification et de préparation de nouveaux ligands stables et d'affinité élevée d'APSM au moyen de la méthode SELEX~ avec des acides nucléiques 2'-O-méthyle substitués et la surface cellulaire SELEX~. L'invention concerne également des méthodes et des compositions de traitement et de diagnostic de maladies caractérisées par l'expression d'APSM au moyen des ligands d'acide nucléique décrits.

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