Stabilized aqueous steroid immunoassay standards

Details Stabilized aqueous steroid immunoassay standards Stabilized aqueous steroid immunoassay standards

G - Physics

01

N

G01N 33/74 (2006.01) G01N 33/96 (2006.01)

Patent

CA 2204027

Aqueous solutions of steroid compounds which have biological activity and have a tendency to oxidative degradation at temperatures between 2 and 8 °C on storage in excess of several months are stabilized by the addition of chelators. Aqueous solutions of particular interest are protein containing solutions which mimic the behavior of human bodily fluids such as serum and are therefore suitable as standards for immunoassays for steroids in such bodily fluids. Chelators of particular interest are transition metal chelators especially those which efficiently sequester iron.

Des solutions aqueuses de composés stéroïdiques ayant une activité biologique et une tendance à la dégradation par oxydation, stockées pendant plusieurs mois à une température comprise entre 2 et 8 DEG C, sont stabilisées par addition de chélateurs. Certaines solutions aqueuses ont un intérêt particulier: ce sont les solutions protéinées qui reproduisent le comportement de liquides organiques tels que le sérum et qui peuvent donc servir d'étalons pour le dosage immunologique des stéroïdes dans de tels liquides. Certains chélateurs ont un intérêt particulier: ce sont les chélateurs des métaux de transition, notamment ceux qui chélatent efficacement le fer.

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