Stabilized bone graft

A - Human Necessities – 61 – F

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A61F 2/28 (2006.01)

Patent

CA 2554571

A demineralized bone matrix (DBM) or other matrix composition is provided that has been stabilized by lowering the pH of the composition, reducing the water content, adding water substitutes, and/or increasing the amount of deuterated water present in the composition in order to reduce the activity of endogenous degrading enzymes such as proteases. A hydrated form of a stabilized DBM composition may be stable up to a year at room temperature at acidic pH. The acidified DBM compositions may be further stabilized by the addition of a stabilizing agent such as deuterated water, water substitutes, polymers, protease inhibitors, glycerol, hydrogels, etc. The invention also provides methods of preparing, testing, and using the inventive stabilized osteodinductive matrix compositions.

L'invention concerne une matrice osseuse déminéralisée (DBM) ou autre composition de matrice stabilisée par diminution du pH de la composition, réduction de la teneur en eau, adjonction de substituts d'eau, et/ou augmentation de la quantité d'eau deutérée présente dans la composition pour diminuer l'activité d'enzymes de dégradation endogènes du type protéase. Une forme hydratée de composition DBM stabilisée peut être stable jusqu'à un an à température ambiante avec pH acide. Les compositions DBM acidifiées peuvent être encore stabilisées par l'adjonction d'agent de stabilisation, du type eau deutérée, substituts d'eau, polymères, inhibiteurs de protéase, glycérol, hydrogels, etc. L'invention concerne également des procédés de préparation, d'essai et d'utilisation des compositions stabilisées de matrices à ostéoinduction décrites.

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