Stabilized dispersed enzymes

Details Stabilized dispersed enzymes Stabilized dispersed enzymes

C - Chemistry, Metallurgy

12

N

150/15, 195/35

C12N 11/04 (2006.01) C12P 1/00 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 1291430

ABSTRACT OF THE DISCLOSURE Enzymes are immobilized, stabilized and evenly dispersed without destroying their activity by sequestering them within micropores of a solid carrier structure and filling the pores with a water-immiscible organic liquid, in which the enzyme remains active. The preferred solid carrier is a microporous membrane. The system is manufactured by providing a solid carrier having a microporous surface whose pores contain an aqueous solution, allowing an aqueous solution of enzyme to equilibrate with the solution in the pores, causing a controlled partial collpase of the pores to effectively trap the enzymes, and replacing the liquid in the pores with a water-immiscible organic liquid. The system can be used to assay for or produce an organic compound. It also can be used for slow release of an enzyme.

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