Stabilized enzyme compositions

Details Stabilized enzyme compositions Stabilized enzyme compositions

C - Chemistry, Metallurgy

12

N

150/15, 150/3.3

C12N 9/12 (2006.01) C12N 9/96 (2006.01) C12Q 1/00 (2006.01) C12Q 1/25 (2006.01) C12Q 1/50 (2006.01)

Patent

CA 2028593

The present invention provides stable, non-lyophilized, enzyme reagent compositions for use in diagnostic assays. A reagent matrix for stabilizing creatine kinase includes at least one enzyme substrate and/or at least one enzyme product combined with the enzyme in a liquid medium. The reagent matrix can also include an enzyme activator chosen from a sulfhydryl compound or a divalent metal ion. The stabilized enzyme reagents of the present invention have an increased applicability to automated instrumentation, as well as, an increased convenience of use in manual assays, without reagent waste due to limited shelf-life.

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