Stabilized enzyme compositions for nucleic acid amplification

Details Stabilized enzyme compositions for nucleic acid amplification Stabilized enzyme compositions for nucleic acid amplification

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01) C12N 9/96 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2210584

Stabilized enzyme compositions for use in nucleic acid amplification. Compositions are provided for the stabilization of one or more enzymes in a single stabilized formulation. Additional compositions incorporate a dried, stabilized enzyme mixture together with necessary cofactors and enzyme substrates in a single container for use upon rehydration. Also disclosed are methods for making and using stabilized enzyme compositions and kits for nucleic acid amplification incorporating the disclosed compositions.

Compositions d'enzymes stabilisées s'utilisant pour effectuer l'amplification d'acide nucléique. Des compositions servent à stabiliser un ou plusieurs enzymes dans une seule formulation stabilisée. D'autres compositions renferment un mélange d'enzymes séchées et stabilisées avec des cofacteurs et des substrats d'enzymes nécessaires dans un seul réservoir afin d'utiliser ledit mélange réhydraté. L'invention concerne également des procédés de préparation et d'utilisation de compositions d'enzymes stabilisées, ainsi que des nécessaires d'amplification d'acide nucléique renfermant lesdites compositions.

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