Stabilized, metal-ion activated l-arginase(l-arginine...

Details Stabilized, metal-ion activated l-arginase(l-arginine... Stabilized, metal-ion activated l-arginase(l-arginine...

C - Chemistry, Metallurgy

12

N

C12N 9/78 (2006.01) C12N 9/96 (2006.01) C12P 13/10 (2006.01)

Patent

CA 2070898

ABSTRACT Stabilized, metal ion activated L-arginase (L-arginine amidinohydrolase; E.C. 3.5.3.1.) is disclosed. Arginase formulations, e.g., for the production of L-ornithine from L-arginine, have the disadvantage that only a small fraction of the enzyme can be recovered after a successful reaction and this, together with the high enzyme costs, renders industrial production of L-ornithine in this way uneconomical. Using the present invention an enzyme stabilized arginase formulation can be made available, so that L-ornithine can be produced with significantly smaller enzyme consumption figures. The stabilization of the arginase formulation is achieved by the addition of a reducing agent, which, it is intended, should be present at at least a 10-fold molar quantity relative to the arginase. The enzymatic reduction of L-ornithine from L-arginine and arginase kits for this purpose.

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